“Development of a method for monitoring the sporulation of Clostridium perfringens”
Clostridium perfringens is an important pathogen associated with food-borne diseases in human. Clinical cases are often associated with school-served lunch and catered foods, which mostly results in large-scale incidents. Clostridium perfringens enterotoxin, a diarrheagenic toxin, is produced in intestinal tract during bacterial sporulation. Therefore, understanding the mechanism how C. perfringens sporulates in the intestine should be an important issue for developing a precautionary measure. Usually, bacterial sporulation is assessed by laborious work, such as by counting viable cells after heat treatment, or by direct counting of spores under microscopy. In this study, we tried to construct a unique reporter system for detection of C. perfringens spore formation using fluorescent protein, where the fluorescence is specifically induced during bacterial sporulation. This system has potential to easily detect sporulating cells and can be used for a large-scale screening of sporulation events.