・Daluthgamage Patsy Himali Wijesekera (Department of Advanced Pathobiology, Osaka Prefecture University)
“Enhancement of anti-tumor immune responses by transfection of cytokine gene into tumor”
Cancer is one of the leading causes of deaths in the world. Despite the advancement in cancer therapy, cancer related deaths are still increasing. Cancer immunotherapy, the manipulation of the patient’s own immune system to combat against tumor cells is now considered as a promising way to treat cancer. However, tumor microenvironment can be a crucial barrier to the success of the cancer immunotherapy. Accordingly, we have used a gene transfection technique with a novel synthetic vector to generate a favorable microenvironment which potentiates the dendritic cell (DC) immunotherapy. There we used canine IFN gamma cytokine gene in vitro transfection to equip the DCs and other immune cells for cytotoxicity against tumor cells. We could show the ability of transfected IFN gamma gene in induction of DC maturation and enhancement of the cytotoxicity against tumor cells. Therefore, we can use this data for the betterment of future cancer immunotherapy in animals and humans.